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BACKGROUND The rate of determining the sequence of the four nucleotides in nucleic acid samples is a majo T technical obstacle for further advancement of molecular biology, medicine, and biotechnology.differentially labelled nucleic acid fragments by degradation at specific nucleotides, or by dideoxy chain teπnination of replicating strands.

Resulting nucleic acid fragments in the range of 1 to 500 bp are then separated on a gel to produce a ladder of bands wherein the adjacent samples differ in length by one nucleotide.

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