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BACKGROUND The rate of determining the sequence of the four nucleotides in nucleic acid samples is a majo T technical obstacle for further advancement of molecular biology, medicine, and biotechnology.differentially labelled nucleic acid fragments by degradation at specific nucleotides, or by dideoxy chain teπnination of replicating strands.

The array-based approach of SBH does not require single base resolution in separation, degradation, synthesis or imaging of a nucleic acid molecule.

Sequence for the target nucleic acid may be assembled by uniquely overlapping scored oligonucleotides.

There are several approaches available to achieve sequencing by hybridization.

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